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Anti-NECTIN4 Polyclonal Antibody (HV985014)

Anti-NECTIN4 Polyclonal Antibody
Anti-NECTIN4 Polyclonal Antibody
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Overview
Catalog No.HV985014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human NECTIN4 (Ala31-Val317).
Target LNIR, NECTIN4, PRR4 receptor-related protein 4, Nectin cell adhesion molecule 4, Ig superfamily receptor LNIR, Nectin-4, PVRL4
Purification Purified by antigen affinity column.
Accession Q96NY8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nectin-4 (NECTIN4/PVRL4) is a ~55 kDa protein. Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells. NECTIN4 is the therapeutic target of enfortumab vedotin (Padcev).

Note For research use only.
Images
  • Anti-NECTIN4 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NECTIN4 polyclonal antibody at 1ug/mL on recombinant NECTIN4 (Catalog No. HV985012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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