Anti-NEFL Polyclonal Antibody (HY123014)

Name: Anti-NEFL Polyclonal Antibody
Brand: abinScience
SKU: HY123014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY123014

Description

Anti-NEFL Polyclonal Antibody (HY123014) is a rabbit polyclonal antibody detecting NEFL in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NEFL (Met1-Asn352).

Target

NEFL, Neurofilament light polypeptide, 68 kDa neurofilament protein, NF68, Neurofilament triplet L protein, NF-L, NFL

Purification

Purified by antigen affinity column.

Accession

P07196

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Neurofilament light polypeptide (NEFL) is a ~61 kDa protein. Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-NEFL polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse cerebellum lysate
Lane 3: Rat brain lysate
Lane 4: Rat cerebellum lysate

References

Datasheet

Anti-NEFL Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-NEFL Polyclonal Antibody (HY123014)

Overview

Images

References

Datasheet

SDS

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