Anti-IkB-alpha Polyclonal Antibody (HB155014)

Name: Anti-IkB-alpha Polyclonal Antibody
Brand: abinScience
SKU: HB155014
Price: 96 USD
Availability: InStock

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SDS

Overview

Catalog No.

HB155014

Description

Anti-NFKBIA Polyclonal Antibody (HB155014) is a rabbit polyclonal antibody detecting NFKBIA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human NFKBIA (Lys67-Glu302).

Target

I-kappa-B-alpha, IKBA, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P25963

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

NF-kappa-B inhibitor alpha (NFKBIA) is a ~35 kDa protein. Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals. On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

1. Ganchi, PA. et al. (1992) Molecular biology of the cell 3, 1339-52. PMID: 1493333

2. Quan, J. et al. (2023) Journal of immunology (Baltimore, Md. : 1950) 210, 640-652. PMID: 36651806

3. Scherer, DC. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 11259-63. PMID: 7479976

4. Chen, Z. et al. (1995) Genes & development 9, 1586-97. PMID: 7628694

5. Traenckner, EB. et al. (1995) The EMBO journal 14, 2876-83. PMID: 7796813

6. Brown, K. et al. (1995) Science (New York, N.Y.) 267, 1485-8. PMID: 7878466

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-NFKBIA polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate

References

Datasheet

Anti-IkB-alpha Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-IkB-alpha Polyclonal Antibody (HB155014)

Overview

Images

References

Datasheet

SDS

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