Anti-NFKBIA Polyclonal Antibody (HB155014)

Name: Anti-NFKBIA Polyclonal Antibody
Brand: abinScience
SKU: HB155014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
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Overview

Catalog No.

HB155014

Description

Anti-NFKBIA Polyclonal Antibody (HB155014) is a rabbit polyclonal antibody detecting NFKBIA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NFKBIA (Lys67-Glu302).

Target

NFKBI, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha, I-kappa-B-alpha, IKBA, NF-kappa-B inhibitor alpha, NFKBIA, MAD3

Purification

Purified by antigen affinity column.

Accession

P25963

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

NF-kappa-B inhibitor alpha (NFKBIA) is a ~35 kDa protein. Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals. On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

1. Ganchi, PA. et al. (1992) Molecular biology of the cell 3, 1339-52. PMID: 1493333

2. Quan, J. et al. (2023) Journal of immunology (Baltimore, Md. : 1950) 210, 640-652. PMID: 36651806

3. Scherer, DC. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 11259-63. PMID: 7479976

4. Chen, Z. et al. (1995) Genes & development 9, 1586-97. PMID: 7628694

5. Traenckner, EB. et al. (1995) The EMBO journal 14, 2876-83. PMID: 7796813

6. Brown, K. et al. (1995) Science (New York, N.Y.) 267, 1485-8. PMID: 7878466

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-NFKBIA polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate

References

Datasheet

Anti-NFKBIA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-NFKBIA Polyclonal Antibody (HB155014)

Overview

Images

References

Datasheet

SDS

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