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Anti-NFKBIE Polyclonal Antibody (HT244014)

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Overview
Catalog No.HT244014
Description
Anti-NFKBIE Polyclonal Antibody (HT244014) is a rabbit polyclonal antibody detecting NFKBIE in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human NFKBIE (Arg207-Pro440).
Target IkB-E, IKBE, IkappaBepsilon, I-kappa-B-epsilon, IkB-epsilon, NFKBIE, NF-kappa-B inhibitor epsilon, NF-kappa-BIE
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O00221
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

NF-kappa-B inhibitor epsilon (NFKBIE) is a ~52 kDa protein. Sequesters NF-kappa-B transcription factor complexes in the cytoplasm, thereby inhibiting their activity. Sequestered complexes include NFKB1-RELA (p50-p65) and NFKB1-REL (p50-c-Rel) complexes. Limits B-cell activation in response to pathogens, and also plays an important role in B-cell development.

1. Li, Z. et al. (1997) Molecular and cellular biology 17, 6184-90. PMID: 9315679
2. Whiteside, ST. et al. (1997) The EMBO journal 16, 1413-26. PMID: 9135156
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-NFKBIE Polyclonal Antibody

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