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Anti-NGB Polyclonal Antibody (HV356014)

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Overview
Catalog No.HV356014
Description
Anti-NGB Polyclonal Antibody (HV356014) is a rabbit polyclonal antibody detecting NGB in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human NGB (Met1-Glu151).
Target NGB, Neuroglobin
Purification Purified by antigen affinity column.
Accession Q9NPG2
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Neuroglobin (NGB) is a ~16 kDa protein. Monomeric globin with a bis-histidyl six-coordinate heme-iron atom through which it can bind dioxygen, carbon monoxide and nitric oxide. Could help transport oxygen and increase its availability to the metabolically active neuronal tissues, though its low quantity in tissues as well as its high affinity for dioxygen, which may limit its oxygen-releasing ability, argue against it. The ferrous/deoxygenated form exhibits a nitrite reductase activity and it could produce nitric oxide which in turn inhibits cellular respiration in response to hypoxia. In its ferrous/deoxygenated state, it may also exhibit GDI (Guanine nucleotide Dissociation Inhibitor) activity toward heterotrimeric G-alpha proteins, thereby regulating signal transduction to facilitate neuroprotective responses in the wake of hypoxia and associated oxidative stress.

1. Dewilde, S. et al. (2001) The Journal of biological chemistry 276, 38949-55. PMID: 11473128
2. Pesce, A. et al. (2003) Structure (London, England : 1993) 11, 1087-95. PMID: 12962627
3. Guimarães, BG. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 1005-14. PMID: 24699645
4. Wakasugi, K. et al. (2003) The Journal of biological chemistry 278, 36505-12. PMID: 12860983
5. Tiso, M. et al. (2011) The Journal of biological chemistry 286, 18277-89. PMID: 21296891
6. Watanabe, S. et al. (2008) Biochemical and biophysical research communications 369, 695-700. PMID: 18302932
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-NGB Polyclonal Antibody

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