Anti-NME5 Polyclonal Antibody (HX241014)

Name: Anti-NME5 Polyclonal Antibody
Brand: abinScience
SKU: HX241014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX241014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NME5 (Ile3-Lys194).

Target

nm23-H5, Testis-specific nm23 homolog, NDK-H 5, Inhibitor of p53-induced apoptosis-beta, NME5, NDP kinase homolog 5, IPIA-beta, Nucleoside diphosphate kinase homolog 5

Purification

Purified by antigen affinity column.

Accession

P56597

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nucleoside diphosphate kinase 5 (NME5) is a ~24 kDa protein. Functions as part of axonemal radial spoke complexes, which play an important part in the motility of sperm and cilia. In vitro, does not display nucleoside diphosphate kinase (NDPK) activity (Probable). Exhibits a 3'-5'-DNA exonuclease activity that removes single nucleotides from the 3' terminus of single-stranded DNA substrates and digests overhanging mismatched 3' termini from double-stranded DNA substrates, possibly participating in DNA nucleolytic processing. Confers protection from cell death mediated by BAX and alters the cellular levels of several antioxidant enzymes, including GPX5. Might be involved in spermiogenesis by enhancing the ability of late-stage spermatids to eliminate reactive oxygen species.

1. Yoon, JH. et al. (2005) Biochemistry 44, 15774-86. PMID: 16313181

2. Munier, A. et al. (1998) FEBS letters 434, 289-94. PMID: 9742940

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-NME5 polyclonal antibody at 1ug/mL on recombinant NME5 (Catalog No. HX241012).

References

Datasheet

Anti-NME5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-NME5 Polyclonal Antibody (HX241014)

Overview

Images

References

Datasheet

SDS

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