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Anti-Norovirus 3CLpro Polyclonal Antibody (VK441024)

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Overview
Catalog No.VK441024
Description
Anti-Norovirus 3CLpro Polyclonal Antibody (VK441024) is a rabbit polyclonal antibody detecting Norovirus 3CLpro in ELISA, IHC, WB. Suitable for Norovirus and Southampton virus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityNorwalk virus (NoV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Norovirus 3CLpro (Ala1101-Glu1281).
Target Chymotrypsin-like cysteine proteinase, Chymotrypsin-like cysteine proteinase
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q83883, Q04544
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Genome polyprotein (3CLpro) is a ~198 kDa protein. Induces the proliferation of the host smooth ER membranes forming long tubular structures. These remodeled membranes probably form the viral factories that contain the replication complex. Induces the disassembly of host Golgi. May play a role in viral replication by interacting with host VAPA, a vesicle-associated membrane protein that plays a role in SNARE-mediated vesicle fusion. This interaction may target replication complex to intracellular membranes (Probable).

1. Fernandez-Vega, V. et al. (2004) Journal of virology 78, 4827-37. PMID: 15078964
Note For research use only
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Formula
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Anti-Norovirus 3CLpro Polyclonal Antibody

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