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Anti-Norovirus VP1/P domain Recombinant Antibody (5I2) (VK441053)

Anti-Norovirus VP1/P domain Recombinant Antibody (5I2)
Anti-Norovirus VP1/P domain Recombinant Antibody (5I2)
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Overview
Catalog No.VK441053
Species reactivityNorwalk virus (NoV)
ApplicationsELISA, SPR
Host speciesHuman
ClonalityMonoclonal
IsotypeIgG1
Clone ID5I2
Target Capsid protein VP1, CP, p59, Soluble capsid protein, Protein 30k, p30, ORF2
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q83884
RRID

Anti-Norovirus/Norwalk virus Capsid protein VP1/p59 Antibody (5I2) (abinScience Cat# VK441053, RRID:AB_3729209)
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Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Capsid protein VP1 is a ~56 kDa protein. Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulates the genomic RNA and is decorated with VP2 proteins. Attaches virion to target cells by binding histo-blood group antigens (HBGAs) present on gastroduodenal epithelial cells.

1. Prasad, BV. et al. (1999) Science (New York, N.Y.) 286, 287-90. PMID: 10514371
2. Jung, J. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 12828-12832. PMID: 31182604
3. White, LJ. et al. (1997) Journal of virology 71, 8066-72. PMID: 9311906
4. Marionneau, S. et al. (2002) Gastroenterology 122, 1967-77. PMID: 12055602
5. Huang, P. et al. (2003) The Journal of infectious diseases 188, 19-31. PMID: 12825167
6. Tan, M. et al. (2006) Journal of virology 80, 7322-31. PMID: 16840313
Note For research use only.
Images
  • Anti-Norovirus VP1/P domain Recombinant Antibody (5I2)

    SDS-PAGE

    SDS-PAGE for Anti-Norovirus/Norwalk virus Capsid protein VP1/p59 Antibody (5I2).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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