Anti-NOS3 Polyclonal Antibody (HB189014)

Name: Anti-NOS3 Polyclonal Antibody
Brand: abinScience
SKU: HB189014
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB189014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NOS3 (Arg756-Gly1161).

Target

Constitutive NOS, NOSIII, eNOS, Endothelial NOS, EC-NOS, Nitric oxide synthase, endothelial, NOS type III, NOS3, cNOS

Purification

Purified by antigen affinity column.

Accession

P29474

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nitric oxide synthase 3 (NOS3) is a ~133 kDa protein. Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

1. Janssens, SP. et al. (1992) The Journal of biological chemistry 267, 14519-22. PMID: 1378832

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-NOS3 polyclonal antibody at 1ug/mL on recombinant NOS3 (Catalog No. HB189022).

References

Datasheet

Anti-NOS3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-NOS3 Polyclonal Antibody (HB189014)

Overview

Images

References

Datasheet

SDS

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