Anti-NPC1 Polyclonal Antibody (HT011024)

Name: Anti-NPC1 Polyclonal Antibody
Brand: abinScience
SKU: HT011024
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT011024

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NPC1 (Arg372-Phe622).

Target

NPC intracellular cholesterol transporter 1, Niemann-Pick C1 protein, NPC1

Purification

Purified by antigen affinity column.

Accession

O15118

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

NPC intracellular cholesterol transporter 1 (NPC1) is a ~142 kDa protein. Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals (Probable).

1. Davies, JP. et al. (2000) The Journal of biological chemistry 275, 24367-74. PMID: 10821832

2. Blom, TS. et al. (2003) Human molecular genetics 12, 257-72. PMID: 12554680

3. Infante, RE. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 15287-92. PMID: 18772377

4. Gong, X. et al. (2016) Cell 165, 1467-1478. PMID: 27238017

5. Carstea, ED. et al. (1997) Science (New York, N.Y.) 277, 228-31. PMID: 9211849

6. Watari, H. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 805-10. PMID: 9927649

7. Kwon, HJ. et al. (2009) Cell 137, 1213-24. PMID: 19563754

8. Ebrahimi-Fakhari, D. et al. (2016) Human molecular genetics 25, 3588-3599. PMID: 27378690

Note

For research use only.

Images

References

Datasheet

Anti-NPC1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-NPC1 Polyclonal Antibody (HT011024)

Overview

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References

Datasheet

SDS

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