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Anti-NSF Polyclonal Antibody (HW407014)

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Overview
Catalog No.HW407014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human NSF (Asp590-Asp744).
Target NSF, NEM-sensitive fusion protein, Vesicle-fusing ATPase, Vesicular-fusion protein NSF, N-ethylmaleimide-sensitive fusion protein
Purification Purified by antigen affinity column.
Accession P46459
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Vesicle-fusing ATPase (NSF) is a ~82 kDa protein. Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.

Note For research use only.
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Formula
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Anti-NSF Polyclonal Antibody

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