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Anti-NSP5/3CL-PRO/M Pro Polyclonal Antibody (VK635014)

Anti-NSP5/3CL-PRO/M Pro Polyclonal Antibody
Anti-NSP5/3CL-PRO/M Pro Polyclonal Antibody
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  • 100ug
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  • Datasheet

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Overview
Catalog No.VK635014
Description
Anti-NSP5/3CL-PRO/M Pro Polyclonal Antibody (VK635014) is a rabbit polyclonal antibody detecting 3CL-PRO in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivitySARS-CoV-2 (2019-nCoV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant SARS-CoV-2 NSP5/3CL-PRO/M Pro (Ser3264-Thr3567).
Target 3C-like proteinase, 3CL-PRO, 3CLp, Main protease, Mpro, Non-structural protein 5, nsp5, SARS coronavirus main proteinase, Replicase polyprotein 1ab, pp1ab, ORF1ab polyprotein
Purification Purified by antigen affinity column.
Accession P0DTD1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Replicase polyprotein 1ab (NSP5/3CL-PRO) is a ~794 kDa protein. Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.

Note For research use only.
Images
  • Anti-NSP5/3CL-PRO/M Pro Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NSP5/3CL-PRO/M Pro polyclonal antibody at 1ug/mL on recombinant NSP5/3CL-PRO/M Pro (Catalog No. VK635022).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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