Anti-NSUN2 Polyclonal Antibody (HC621014)

Name: Anti-NSUN2 Polyclonal Antibody
Brand: abinScience
SKU: HC621014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HC621014

Description

Anti-NSUN2 Polyclonal Antibody (HC621014) is a rabbit polyclonal antibody detecting NSUN2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NSUN2 (His415-Arg767).

Target

Misu, NSUN2, hTrm4, RNA cytosine C(5)-methyltransferase NSUN2, TRM4, NOL1/NOP2/Sun domain family member 2, tRNA methyltransferase 4 homolog, mRNA cytosine C(5)-methyltransferase, SAKI, Substrate of AIM1/Aurora kinase B, Myc-induced SUN domain-containing protein, tRNA cytosine C(5)-methyltransferase

Purification

Purified by antigen affinity column.

Accession

Q08J23

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

RNA cytosine C(5)-methyltransferase NSUN2 is a ~86 kDa protein. RNA cytosine C(5)-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development: acts by increasing protein synthesis; cytosine C(5)-methylation promoting tRNA stability and preventing mRNA decay. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs). Also mediates C(5)-methylation of mitochondrial tRNAs.

1. Brzezicha, B. et al. (2006) Nucleic acids research 34, 6034-43. PMID: 17071714

2. Auxilien, S. et al. (2012) RNA biology 9, 1331-8. PMID: 22995836

3. Gkatza, NA. et al. (2019) PLoS biology 17, e3000297. PMID: 31199786

4. Chen, X. et al. (2019) Nature cell biology 21, 978-990. PMID: 31358969

5. Van Haute, L. et al. (2019) Nucleic acids research 47, 8720-8733. PMID: 31276587

9. Hussain, S. et al. (2013) Cell reports 4, 255-61. PMID: 23871666

Note

For research use only.

Images

References

Datasheet

Anti-NSUN2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-NSUN2 Polyclonal Antibody (HC621014)

Overview

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References

Datasheet

SDS

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