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Anti-NT5C1A/cN1A Polyclonal Antibody (HA587014)

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Overview
Catalog No.HA587014
Description
Anti-NT5C1A/cN1A Polyclonal Antibody (HA587014) is a rabbit polyclonal antibody detecting NT5C1A in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human NT5C1A/cN1A (Met1-Gln368).
Target Cytosolic 5'-nucleotidase 1A, cN1A, EC:3.1.3.5, EC:3.1.3.89, EC:3.1.3.99, 5'-deoxynucleotidase, Cytosolic 5'-nucleotidase IA, cN-I, cN-IA, NT5C1A
Endotoxin level Please contact with the lab for this information.
Observed Molecular Weight ~42 kDa
Purification Purified by antigen affinity column.
Accession Q9BXI3, A3KFX0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Cytosolic 5'-nucleotidase 1A (NT5C1A/cN1A) is a ~41 kDa protein. Catalyzes the hydrolysis of ribonucleotide and deoxyribonucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside. AMP is the major substrate but can also hydrolyze dCMP and IMP.

1. Hunsucker, SA. et al. (2001) The Journal of biological chemistry 276, 10498-504. PMID: 11133996
2. Jędrzejewska, A. et al. (2022) Nucleosides, nucleotides & nucleic acids 41, 273-284. PMID: 34814800
3. Tavenier, M. et al. (1995) Biochimica et biophysica acta 1244, 351-6. PMID: 7599155
4. Skladanowski, AC. et al. (1996) The American journal of physiology 270, H1493-500. PMID: 8967393
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-NT5C1A/cN1A Polyclonal Antibody

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