Anti-NTMT2 Polyclonal Antibody (HB306014)

Name: Anti-NTMT2 Polyclonal Antibody
Brand: abinScience
SKU: HB306014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB306014

Description

Anti-NTMT2 Polyclonal Antibody (HB306014) is a rabbit polyclonal antibody detecting N-terminal Xaa-Pro-Lys N-methyltransferase 2; 2.1.1.299; Alpha N-terminal protein methyltransferase 1B; Methyltransferase-like protein 11B; X-Pro-Lys N-terminal protein methyltransferase 1B; NTM1B; NTMT2; C1orf184; METTL11B; NRMT2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human NTMT2 (Met1-Ser283).

Target

N-terminal Xaa-Pro-Lys N-methyltransferase 2; 2.1.1.299; Alpha N-terminal protein methyltransferase 1B; Methyltransferase-like protein 11B; X-Pro-Lys N-terminal protein methyltransferase 1B; NTM1B; NTMT2; C1orf184; METTL11B; NRMT2

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q5VVY1, B2RXM4, D3ZVR1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

N-terminal Xaa-Pro-Lys N-methyltransferase 2 (NTMT2) is a ~32 kDa protein. Alpha N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes monomethylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and Pro in the Pro-Pro-Lys motif. Predominantly functions as a mono-methyltransferase but is also able to di-/tri-methylate the GPKRIA peptide and di-methylate the PPKRIA peptide (in vitro). May activate NTMT1 by priming its substrates for trimethylation.

1. Petkowski, JJ. et al. (2013) The Biochemical journal 456, 453-62. PMID: 24090352

2. Dong, C. et al. (2018) Communications biology 1, 183. PMID: 30417120

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-NTMT2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse heart lysate
Lane 2: Rat heart lysate

References

Datasheet

Anti-NTMT2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-NTMT2 Polyclonal Antibody (HB306014)

Overview

Images

References

Datasheet

SDS

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