Anti-Nucleoprotein Polyclonal Antibody (VK432024)

Name: Anti-Nucleoprotein Polyclonal Antibody
Brand: abinScience
SKU: VK432024
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK432024

Description

Anti-Nucleoprotein Polyclonal Antibody (VK432024) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB. Suitable for Hendra virus and Nipah virus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Hendra virus (HeV), Nipah virus (NiV)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant hendra virus Nucleoprotein (Met1-Ala394).

Target

nucleocapsid, N

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q9IK92, O89339

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nucleoprotein is a ~58 kDa protein. Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. The nucleocapsid (NC) has a helical structure with either 13.4 N per turn, approximately 20 nm in diameter, with a hollow central cavity of 56 nm in diameter. The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P.

1. Ker, DS. et al. (2021) PLoS pathogens 17, e1009740. PMID: 34270629

2. Yabukarski, F. et al. (2014) Nature structural & molecular biology 21, 754-9. PMID: 25108352

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-Nucleocapsid-Hendra virus polyclonal antibody at 1ug/mL on various samples.
Lane 1: ATG18514 100ng

References

Datasheet

Anti-Nucleoprotein Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Nucleoprotein Polyclonal Antibody (VK432024)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us