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Anti-NUP62 Polyclonal Antibody (MW461014)

Anti-NUP62 Polyclonal Antibody
Anti-NUP62 Polyclonal Antibody
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Overview
Catalog No.MW461014
Description
Anti-NUP62 Polyclonal Antibody (MW461014) is a rabbit polyclonal antibody detecting Nuclear pore glycoprotein p62; 62 kDa nucleoporin; Nucleoporin Nup62; Nup62 in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant mouse NUP62 (Gln428-Asp526).
Target Nuclear pore glycoprotein p62; 62 kDa nucleoporin; Nucleoporin Nup62; Nup62
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P17955, Q63850, P37198
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nuclear pore glycoprotein p62 (NUP62) is a ~53 kDa protein. Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex. Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation. It might be involved in protein recruitment to the centrosome after nuclear breakdown.

1. Starr, CM. et al. (1990) The Journal of cell biology 110, 1861-71. PMID: 2190987
2. Hu, T. et al. (1996) The Journal of cell biology 134, 589-601. PMID: 8707840
Note For research use only
Images
  • Anti-NUP62 Polyclonal Antibody

    Western blot

    Lane 1: NIH3T3 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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