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Anti-NUP98 Polyclonal Antibody (HW311014)

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Overview
Catalog No.HW311014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human NUP98 (Glu629-Glu894).
Target 98 kDa nucleoporin, Nucleoporin Nup98, Nup98, Nuclear pore complex protein Nup98-Nup96, Nup96, Nucleoporin Nup96, ADAR2, 96 kDa nucleoporin, NUP98
Purification Purified by antigen affinity column.
Accession P52948
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nuclear pore complex protein Nup98-Nup96 is a ~197 kDa protein. Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes. Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body).

1. Miorin, L. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 28344-28354. PMID: 33097660
2. Capitanio, JS. et al. (2017) eLife 6. PMID: 28221134
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-NUP98 Polyclonal Antibody

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