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Anti-ORF7a/Protein U122 Polyclonal Antibody (VK586014)

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Overview
Catalog No.VK586014
Description
Anti-ORF7a/Protein U122 Polyclonal Antibody (VK586014) is a rabbit polyclonal antibody detecting ORF7a protein in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivitySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant SARS-CoV-2 ORF7a/Protein U122 (Glu16-Tyr97).
Target ORF7a protein, ORF7a, Accessory protein 7a, Protein U122, Protein X4, 7a
Purification Purified by antigen affinity column.
Accession P0DTC7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

ORF7a protein (Protein U122) is a ~13 kDa protein. Plays a role as antagonist of host tetherin (BST2), disrupting its antiviral effect. Acts by binding to BST2 and sequestering it to perinuclear region, thereby preventing its antiviral function at cell membrane. May specifically downregulate MHC-I allele HLA-A*02:01 (HLA-A2).

1. Martin-Sancho, L. et al. (2021) Molecular cell 81, 2656-2668.e8. PMID: 33930332
2. Arshad, N. et al. (2023) Proceedings of the National Academy of Sciences of the United States of America 120, e2208525120. PMID: 36574644
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-ORF7a/Protein U122 Polyclonal Antibody

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