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Anti-OTUD3 Polyclonal Antibody (HA837014)

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Overview
Catalog No.HA837014
Description
Anti-OTUD3 Polyclonal Antibody (HA837014) is a rabbit polyclonal antibody detecting OTU domain-containing protein 3; 3.4.19.12; OTUD3; KIAA0459 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human OTUD3 (Gly46-Lys220).
Target OTU domain-containing protein 3; 3.4.19.12; OTUD3; KIAA0459
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q5T2D3, B1AZ99
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

OTU domain-containing protein 3 (OTUD3) is a ~45 kDa protein. Deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. Also hydrolyzes heterotypic (mixed and branched) and homotypic chains. Important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation.

1. Mevissen, TE. et al. (2013) Cell 154, 169-84. PMID: 23827681
2. Garshott, DM. et al. (2020) eLife 9. PMID: 32011234
3. Zhou, N. et al. (2022) Cell metabolism 34, 1023-1041.e8. PMID: 35675826
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-OTUD3 Polyclonal Antibody

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