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Anti-PACC1 Polyclonal Antibody (HB482014)

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Overview
Catalog No.HB482014
Description
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human PACC1 (His98-Ala301).
Target Proton-activated chloride channel; PAC; hPAC; Acid-sensitive outwardly-rectifying anion channel; ASOR; Proton-activated outwardly rectifying anion channel; PAORAC; Transmembrane protein 206; hTMEM206; PACC1; C1orf75; TMEM206
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9H813, Q2KHV2, Q66H28, Q9D771
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Proton-activated chloride channel (PACC1) is a ~40 kDa protein. Chloride channel gated by pH that facilitates the entry of chloride ions into cells upon exposure to extracellular acidic pH. Involved in acidosis-induced cell death by mediating chloride influx and subsequent cell swelling.

1. Yang, J. et al. (2019) Science (New York, N.Y.) 364, 395-399. PMID: 31023925
2. Ullrich, F. et al. (2019) eLife 8. PMID: 31318332
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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