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Anti-PAFAH1B2 Polyclonal Antibody (HB338014)

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Overview
Catalog No.HB338014
Description
Anti-PAFAH1B2 Polyclonal Antibody (HB338014) is a rabbit polyclonal antibody detecting Platelet-activating factor acetylhydrolase IB subunit alpha2; 3.1.1.47; PAF acetylhydrolase 30 kDa subunit; PAF-AH 30 kDa subunit; PAF-AH subunit beta; PAFAH subunit beta; PAFAH1B2; PAFAHB in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, Mouse, and Chicken.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human PAFAH1B2 (Met1-Ala229).
Target Platelet-activating factor acetylhydrolase IB subunit alpha2; 3.1.1.47; PAF acetylhydrolase 30 kDa subunit; PAF-AH 30 kDa subunit; PAF-AH subunit beta; PAFAH subunit beta; PAFAH1B2; PAFAHB
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P68402, P68401, O35264, Q61206, Q5ZMS2
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Platelet-activating factor acetylhydrolase IB subunit alpha2 (PAFAH1B2) is a ~25 kDa protein. Alpha2 catalytic subunit of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I)) heterotetrameric enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and modulates the action of PAF. The activity and substrate specificity of PAF-AH (I) are affected by its subunit composition. The alpha2/alpha2 homodimer (PAFAH1B2/PAFAH1B2 homodimer) hydrolyzes PAF and 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylethanolamine (AAGPE) more efficiently than 1-O-alkyl-2-acetyl-sn-glycero-3-phosphoric acid (AAGPA). In contrast, the alpha1/alpha2 heterodimer(PAFAH1B3/PAFAH1B3 heterodimer) hydrolyzes AAGPA more efficiently than PAF, but has little hydrolytic activity towards AAGPE. May play a role in male germ cell meiosis during the late pachytenestage and meiotic divisions as well as early spermiogenesis.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-PAFAH1B2 Polyclonal Antibody

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