Anti-PAM Polyclonal Antibody (HB756014)

Name: Anti-PAM Polyclonal Antibody
Brand: abinScience
SKU: HB756014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HB756014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PAM (Phe21-Cys288).

Target

PAL, PHM, PAM, Peptidylamidoglycolate lyase, Peptidyl-glycine alpha-amidating monooxygenase

Purification

Purified by antigen affinity column.

Accession

P19021

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Peptidyl-glycine alpha-amidating monooxygenase (PAM) is a ~108 kDa protein. Bifunctional enzyme that catalyzes amidation of the C-terminus of proteins. Alpha-amidation is present at the C-terminus of many endocrine hormones and neuropeptides and is required for their activity. C-terminal amidation also takes place in response to protein fragmentation triggered by oxidative stress, promoting degradation of amidated protein fragments by the proteasome. Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monooxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate.

1. Satani, M. et al. (2003) Protein expression and purification 28, 293-302. PMID: 12699694

2. Glauder, J. et al. (1990) Biochemical and biophysical research communications 169, 551-8. PMID: 2357221

3. Eipper, BA. et al. (1992) Annual review of neuroscience 15, 57-85. PMID: 1575450

4. Stadtman, ER. (1990) Biochemistry 29, 6323-31. PMID: 2207077

Note

For research use only.

Images

References

Datasheet

Anti-PAM Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-PAM Polyclonal Antibody (HB756014)

Overview

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References

Datasheet

SDS

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