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Anti-PAPSS2 Polyclonal Antibody (HB592014)

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Overview
Catalog No.HB592014
Description
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human PAPSS2 (Glu231-Asn614).
Target Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2; PAPS synthase 2; PAPSS 2; Sulfurylase kinase 2; SK 2; SK2; Sulfate adenylyltransferase; 2.7.7.4; ATP-sulfurylase; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; 2.7.1.25; 3'-phosphoadenosine-5'-phosphosulfate synthase; APS kinase; Adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenylylsulfate 3'-phosphotransferase; PAPSS2; ATPSK2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O95340, O88428
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (PAPSS2) is a ~69 kDa protein. Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate/PAPS, the activated sulfate donor used by sulfotransferases. In mammals, PAPS is the sole source of sulfate while APS appears to only be an intermediate in the sulfate-activation pathway. Plays indirectly an important role in skeletogenesis during postnatal growth.

1. Xu, ZH. et al. (2002) Pharmacogenetics 12, 11-21. PMID: 11773860
2. Noordam, C. et al. (2009) The New England journal of medicine 360, 2310-8. PMID: 19474428
3. Iida, A. et al. (2013) Human mutation 34, 1381-6. PMID: 23824674
4. Oostdijk, W. et al. (2015) The Journal of clinical endocrinology and metabolism 100, E672-80. PMID: 25594860
5. Faiyaz ul Haque, M. et al. (1998) Nature genetics 20, 157-62. PMID: 9771708
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-PAPSS2 Polyclonal Antibody

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