Anti-PARN Polyclonal Antibody (HF535014)

Name: Anti-PARN Polyclonal Antibody
Brand: abinScience
SKU: HF535014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HF535014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PARN (Lys178-Asp245).

Target

Deadenylating nuclease, Polyadenylate-specific ribonuclease, Poly(A)-specific ribonuclease PARN, Deadenylation nuclease, PARN, DAN

Purification

Purified by antigen affinity column.

Accession

O95453

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Poly(A)-specific ribonuclease PARN is a ~73 kDa protein. 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP.

1. Gao, M. et al. (2000) Molecular cell 5, 479-88. PMID: 10882133

2. Martînez, J. et al. (2001) The Journal of biological chemistry 276, 27923-9. PMID: 11359775

3. Lai, WS. et al. (2003) Molecular and cellular biology 23, 3798-812. PMID: 12748283

4. Gherzi, R. et al. (2004) Molecular cell 14, 571-83. PMID: 15175153

5. Körner, CG. et al. (1998) The EMBO journal 17, 5427-37. PMID: 9736620

6. Berndt, H. et al. (2012) RNA (New York, N.Y.) 18, 958-72. PMID: 22442037

7. Boele, J. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 11467-72. PMID: 25049417

Note

For research use only.

Images

References

Datasheet

Anti-PARN Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PARN Polyclonal Antibody (HF535014)

Overview

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References

Datasheet

SDS

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