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Anti-PCK1 Polyclonal Antibody (HW538014)

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Overview
Catalog No.HW538014
Description
Anti-PCK1 Polyclonal Antibody (HW538014) is a rabbit polyclonal antibody detecting PCK1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human PCK1 (Asp311-Pro559).
Target PCK1, PEPCK1, PEPCK-C, Serine-protein kinase PCK1, Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Purification Purified by antigen affinity column.
Accession P35558
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (PCK1) is a ~69 kDa protein. Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis. Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. Acts as a regulator of formation and maintenance of memory CD8(+) T-cells: up-regulated in these cells, where it generates phosphoenolpyruvate, via gluconeogenesis.

1. Adams, DR. et al. (2014) Molecular genetics and metabolism 113, 161-70. PMID: 24863970
2. Santra, S. et al. (2016) Molecular genetics and metabolism 118, 21-7. PMID: 26971250
3. Vieira, P. et al. (2017) Molecular genetics and metabolism 120, 337-341. PMID: 28216384
4. Latorre-Muro, P. et al. (2018) Molecular cell 71, 718-732.e9. PMID: 30193097
5. Xu, D. et al. (2020) Nature 580, 530-535. PMID: 32322062
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-PCK1 Polyclonal Antibody

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