Anti-PCLAF Polyclonal Antibody (HC630014)

Name: Anti-PCLAF Polyclonal Antibody
Brand: abinScience
SKU: HC630014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC630014

Description

Anti-PCLAF Polyclonal Antibody (HC630014) is a rabbit polyclonal antibody detecting PCLAF in ELISA, IHC, WB. Suitable for Human, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PCLAF (Met1-Glu111).

Target

PAF, Overexpressed in anaplastic thyroid carcinoma 1, PCNA-associated factor of 15 kDa, PCLAF, Hepatitis C virus NS5A-transactivated protein 9, PCNA-associated factor, OEATC-1, PCNA-clamp-associated factor, PAF15, HCV NS5A-transactivated protein 9, p15PAF, NS5ATP9, KIAA0101

Purification

Purified by antigen affinity column.

Accession

Q15004

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

PCNA-associated factor (PCLAF) is a ~11 kDa protein. PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number.

Note

For research use only.

Images

References

Datasheet

Anti-PCLAF Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PCLAF Polyclonal Antibody (HC630014)

Overview

Images

References

Datasheet

SDS

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