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Anti-PCYOX1 Polyclonal Antibody (HV218014)

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Overview
Catalog No.HV218014
Description
Anti-PCYOX1 Polyclonal Antibody (HV218014) is a rabbit polyclonal antibody detecting PCYOX1 in ELISA, IHC, WB. Suitable for Human, Macaca fascicularis, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PCYOX1 (Tyr174-Leu505).
Target Prenylcysteine oxidase 1, Prenylcysteine lyase, PCYOX1, KIAA0908, PCL1
Purification Purified by antigen affinity column.
Accession Q9UHG3
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Prenylcysteine oxidase 1 (PCYOX1) is a ~56 kDa protein. Prenylcysteine oxidase that cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine. Only active against free prenylcysteines and not prenylcysteine residues within prenylated proteins or peptides. Involved in the final step in the degradation of prenylated proteins, by degrading prenylcysteines after the protein has been degraded.

1. Tschantz, WR. et al. (1999) The Journal of biological chemistry 274, 35802-8. PMID: 10585463
2. Tschantz, WR. et al. (2001) The Journal of biological chemistry 276, 2321-4. PMID: 11078725
3. Digits, JA. et al. (2002) The Journal of biological chemistry 277, 41086-93. PMID: 12186880
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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