Anti-PDCD6IP Polyclonal Antibody (HV421014)

Name: Anti-PDCD6IP Polyclonal Antibody
Brand: abinScience
SKU: HV421014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV421014

Description

Anti-PDCD6IP Polyclonal Antibody (HV421014) is a rabbit polyclonal antibody detecting PDCD6IP in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PDCD6IP (Glu174-Ala383).

Target

ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95, AIP1, ALIX, KIAA1375, PDCD6IP, Programmed cell death 6-interacting protein, PDCD6-interacting protein

Purification

Purified by antigen affinity column.

Accession

Q8WUM4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Programmed cell death 6-interacting protein (PDCD6IP) is a ~96 kDa protein. Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis.

1. Matsuo, H. et al. (2004) Science (New York, N.Y.) 303, 531-4. PMID: 14739459

2. Carlton, JG. et al. (2007) Science (New York, N.Y.) 316, 1908-12. PMID: 17556548

3. Morita, E. et al. (2007) The EMBO journal 26, 4215-27. PMID: 17853893

4. Carlton, JG. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 10541-6. PMID: 18641129

5. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PDCD6IP polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: Mouse liver lysate
Lane 6: Rat liver lysate