Anti-PDHA2 Polyclonal Antibody (HA574014)

Name: Anti-PDHA2 Polyclonal Antibody
Brand: abinScience
SKU: HA574014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA574014

Description

Anti-PDHA2 Polyclonal Antibody (HA574014) is a rabbit polyclonal antibody detecting PDHA2 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Rat, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PDHA2 (Ser28-Ser388).

Target

Pyruvate dehydrogenase E1 component subunit alpha testis-specific form mitochondrial, EC:1.2.4.1, PDHE1-A type II, PDHA2

Endotoxin level

Please contact with the lab for this information.

Observed Molecular Weight

~43 kDa

Purification

Purified by antigen affinity column.

Accession

P29803, P35486, , A7MB35, P26284, P29804, P08559

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial (PDHA2) is a ~42 kDa protein. Together with PDHB forms the heterotetrameric E1 subunit of the pyruvate dehydrogenase (PDH) complex in testis. The PDH complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3) (Probable). The E1 subunit catalyzes both the thiamine pyrophosphate (TPP)-dependent decarboxylation of pyruvate and the reductive acetylation of a lipoyl group covalently linked to the lipoyl-bearing domains of E2.

1. Hiromasa, Y. et al. (2004) The Journal of biological chemistry 279, 6921-33. PMID: 14638692

2. Korotchkina, LG. et al. (2006) The Journal of biological chemistry 281, 9688-96. PMID: 16436377

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-PDHA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse kidney lysate
Lane 3: Mouse testis lysate
Lane 4: Rat brain lysate
Lane 5: Rat kidney lysate

References

Datasheet

Anti-PDHA2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PDHA2 Polyclonal Antibody (HA574014)

Overview

Images

References

Datasheet

SDS

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