Anti-PDIA3 Polyclonal Antibody (HB133014)

Name: Anti-PDIA3 Polyclonal Antibody
Brand: abinScience
SKU: HB133014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HB133014

Description

Anti-PDIA3 Polyclonal Antibody (HB133014) is a rabbit polyclonal antibody detecting PDIA3 in WB, IHC, ELISA. Suitable for Human, Mus musculus, Rattus norvegicus, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

WB, IHC, ELISA

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant PDIA3 (Asp162-Tyr416).

Target

PDIA3

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

P30101

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Protein disulfide-isomerase A3, EC:5.3.4.1, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, p58, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, PDIA3

Background

Protein disulfide-isomerase A3 (PDIA3) is a ~56 kDa protein. Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone. Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity.

1. Dick, TP. et al. (2002) Immunity 16, 87-98. PMID: 11825568

2. Peaper, DR. et al. (2005) The EMBO journal 24, 3613-23. PMID: 16193070

3. Gaucci, E. et al. (2016) Scientific reports 6, 37957. PMID: 27897272

4. Müller, IK. et al. (2022) Nature communications 13, 5383. PMID: 36104323

5. Bourdi, M. et al. (1995) Archives of biochemistry and biophysics 323, 397-403. PMID: 7487104

6. Domnick, A. et al. (2022) Nature communications 13, 4701. PMID: 35948544

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PDIA3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: A375 cell lysate
Lane 3: LO2 cell lysate
Lane 4: U2OS cell lysate
Lane 5: Lncap cell lysate
Lane 6: K562 cell lysate
Lane 7: Jurkat cell lysate

References

Datasheet

Anti-PDIA3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PDIA3 Polyclonal Antibody (HB133014)

Overview

Images

References

Datasheet

SDS

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