Anti-PDK1 Polyclonal Antibody (HS822014)

Name: Anti-PDK1 Polyclonal Antibody
Brand: abinScience
SKU: HS822014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HS822014

Description

Anti-PDK1 Polyclonal Antibody (HS822014) is a rabbit polyclonal antibody detecting PDK1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PDK1 (Tyr233-Met430).

Target

PDHK1, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1

Purification

Purified by antigen affinity column.

Accession

Q15118

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1) is a ~49 kDa protein. Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia.

1. Gudi, R. et al. (1995) The Journal of biological chemistry 270, 28989-94. PMID: 7499431

2. McFate, T. et al. (2008) The Journal of biological chemistry 283, 22700-8. PMID: 18541534

3. Hitosugi, T. et al. (2011) Molecular cell 44, 864-77. PMID: 22195962

4. Li, X. et al. (2016) Molecular cell 61, 705-719. PMID: 26942675

5. Kato, M. et al. (2007) Structure (London, England : 1993) 15, 992-1004. PMID: 17683942

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PDK1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: 293T cell lysate
Lane 3: PC-12 cell lysate
Lane 4: Lncap cell lystae
Lane 5: Hela cell lysate