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Anti-PDXK Polyclonal Antibody (HT485014)

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Overview
Catalog No.HT485014
Description
Anti-PDXK Polyclonal Antibody (HT485014) is a rabbit polyclonal antibody detecting PDXK in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Ovis aries, and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human PDXK (Gln29-Lys253).
Target Pyridoxine kinase, Pyridoxal kinase, C21orf97, PDXK, PNK, PKH, C21orf124
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O00764
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Pyridoxal kinase (PDXK) is a ~35 kDa protein. Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.

1. Lee, HS. et al. (2000) Molecules and cells 10, 452-9. PMID: 10987144
2. Musayev, FN. et al. (2007) Protein science : a publication of the Protein Society 16, 2184-94. PMID: 17766369
3. Gandhi, AK. et al. (2009) Biochemical and biophysical research communications 381, 12-5. PMID: 19351586
4. Chelban, V. et al. (2019) Annals of neurology 86, 225-240. PMID: 31187503
5. Hanna, MC. et al. (1997) The Journal of biological chemistry 272, 10756-60. PMID: 9099727
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-PDXK Polyclonal Antibody

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