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Anti-PEDV N/Nucleoprotein Polyclonal Antibody (VK727024)

Anti-PEDV N/Nucleoprotein Polyclonal Antibody
Anti-PEDV N/Nucleoprotein Polyclonal Antibody
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Spec:
  • 50ug
  • 100ug
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  • Datasheet

Overview
Catalog No.VK727024
Species reactivityPorcine epidemic diarrhea virus (strain CV777) (PEDV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant PEDV N/Nucleoprotein (Met1-Asn441).
Target Nucleoprotein, Nucleocapsid protein, NC, Protein N, N, 6
Purification Purified by antigen affinity column.
Accession Q07499
RRID Anti-PEDV N/Nucleoprotein Polyclonal Antibody (abinScience Cat# VK727024, RRID:AB_3723658)
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nucleoprotein is a ~48 kDa protein. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

Note For research use only.
Images
  • Anti-PEDV N/Nucleoprotein Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PEDV N/Nucleoprotein polyclonal antibody at 1ug/mL on recombinant PEDV N/Nucleoprotein (Catalog No. VK727022).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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