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Anti-PEPD Polyclonal Antibody (HD441014)

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Anti-PEPD Polyclonal Antibody
Anti-PEPD Polyclonal Antibody
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Overview
Catalog No.HD441014
Description
Anti-PEPD Polyclonal Antibody (HD441014) is a rabbit polyclonal antibody detecting PEPD in ELISA, IHC, WB. Suitable for Mouse, Rat, and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human PEPD (Thr188-Lys493).
Target EC:3.4.13.9, Imidodipeptidase, PEPD, PRD, Peptidase D, Prolidase, Proline dipeptidase, X-Pro dipeptidase, Xaa-Pro dipeptidase
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P12955
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesP12955
Background

Xaa-Pro dipeptidase (PEPD) is a ~54 kDa protein. Dipeptidase that catalyzes the hydrolysis of dipeptides with a prolyl (Xaa-Pro) or hydroxyprolyl residue in the C-terminal position. The preferred dipeptide substrate is Gly-Pro, but other Xaa-Pro dipeptides, such as Ala-Pro, Met-Pro, Phe-Pro, Val-Pro and Leu-Pro, can be cleaved. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.

1. Lupi, A. et al. (2006) The FEBS journal 273, 5466-78. PMID: 17081196
2. Rao, SD. et al. (2022) Nature chemical biology 18, 565-574. PMID: 35165443
3. Endo, F. et al. (1989) The Journal of biological chemistry 264, 4476-81. PMID: 2925654
Note For research use only
Images
  • Anti-PEPD Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PEPD polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: SH-SY5Y cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-PEPD Polyclonal Antibody

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