Anti-PGAM5 Polyclonal Antibody (HB500014)

Name: Anti-PGAM5 Polyclonal Antibody
Brand: abinScience
SKU: HB500014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB500014

Description

Anti-PGAM5 Polyclonal Antibody (HB500014) is a rabbit polyclonal antibody detecting Serine/threonine-protein phosphatase PGAM5 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human PGAM5 (Ala94-Ser289).

Target

Serine/threonine-protein phosphatase PGAM5, mitochondrial; 3.1.3.16; Bcl-XL-binding protein v68; Phosphoglycerate mutase family member 5; PGAM5

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q96HS1, Q8BX10, Q562B5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine/threonine-protein phosphatase PGAM5, mitochondrial is a ~32 kDa protein. Mitochondrial serine/threonine phosphatase that dephosphorylates various substrates and thus plays a role in different biological processes including cellular senescence or mitophagy. Modulates cellular senescence by regulating mitochondrial dynamics. Mechanistically, participates in mitochondrial fission through dephosphorylating DNM1L/DRP1. Additionally, dephosphorylates MFN2 in a stress-sensitive manner and consequently protects it from ubiquitination and degradation to promote mitochondrial network formation. Regulates mitophagy independent of PARKIN by interacting with and dephosphorylating FUNDC1, which interacts with LC3.

1. Chen, G. et al. (2014) Molecular cell 54, 362-77. PMID: 24746696

2. Yu, B. et al. (2020) Nature communications 11, 2549. PMID: 32439975

3. Nag, S. et al. (2023) Cell reports 42, 112895. PMID: 37498743

4. Lo, SC. et al. (2008) Experimental cell research 314, 1789-803. PMID: 18387606

5. Wang, Z. et al. (2012) Cell 148, 228-43. PMID: 22265414

Note

For research use only

Images

References

Datasheet

Anti-PGAM5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PGAM5 Polyclonal Antibody (HB500014)

Overview

Images

References

Datasheet

SDS