Anti-PGK1 Polyclonal Antibody (HF674014)

Name: Anti-PGK1 Polyclonal Antibody
Brand: abinScience
SKU: HF674014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HF674014

Description

Anti-PGK1 Polyclonal Antibody (HF674014) is a rabbit polyclonal antibody detecting PGK1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Cricetulus griseus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PGK1 (Ser2-Ile417).

Target

PRP 2, Primer recognition protein 2, PGKA, PGK1, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein

Purification

Purified by antigen affinity column.

Accession

P00558

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Phosphoglycerate kinase 1 (PGK1) is a ~44 kDa protein. Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl-coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis. May play a role in sperm motility.

1. Bollong, MJ. et al. (2018) Nature 562, 600-604. PMID: 30323285

2. Fujii, H. et al. (1980) The Journal of biological chemistry 255, 6421-3. PMID: 7391028

3. Gondeau, C. et al. (2008) Nucleic acids research 36, 3620-9. PMID: 18463139

4. Jindal, HK. et al. (1990) The Journal of biological chemistry 265, 6540-3. PMID: 2324090

5. Li, X. et al. (2016) Molecular cell 61, 705-719. PMID: 26942675

6. Chen, Z. et al. (2023) Nature communications 14, 1121. PMID: 36849569

7. Liu, XX. et al. (2016) Human reproduction (Oxford, England) 31, 273-9. PMID: 26677959

Note

For research use only.

Images

References

Datasheet

Anti-PGK1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-PGK1 Polyclonal Antibody (HF674014)

Overview

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References

Datasheet

SDS

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