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Anti-PGM1 Polyclonal Antibody (HW522014)

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Overview
Catalog No.HW522014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PGM1 (Gln15-Lys235).
Target Phosphoglucomutase-1, Glucose phosphomutase 1, PGM 1, PGM1
Purification Purified by antigen affinity column.
Accession P36871
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Phosphoglucomutase-1 (PGM1) is a ~61 kDa protein. Catalyzes the reversible isomerization of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate. The mechanism proceeds via the intermediate compound alpha-D-glucose 1,6-bisphosphate (Probable). This enzyme participates in both the breakdown and synthesis of glucose.

1. Gururaj, A. et al. (2004) Oncogene 23, 8118-27. PMID: 15378030
2. Lee, Y. et al. (2014) The Journal of biological chemistry 289, 32010-32019. PMID: 25288802
3. Yu, LR. et al. (2007) Journal of proteome research 6, 4150-62. PMID: 17924679
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-PGM1 Polyclonal Antibody

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