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Anti-PHB Polyclonal Antibody (HW829014)

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Overview
Catalog No.HW829014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PHB (Lys177-Gln272).
Target Prohibitin, PHB, PHB1
Purification Purified by antigen affinity column.
Accession P35232
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Prohibitin 1 (PHB) is a ~29 kDa protein. Protein with pleiotropic attributes mediated in a cell-compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors in the nucleus. Plays a role in adipose tissue and glucose homeostasis in a sex-specific manner. Contributes to pulmonary vascular remodeling by accelerating proliferation of pulmonary arterial smooth muscle cells.

1. Coates, PJ. et al. (2001) Experimental cell research 265, 262-73. PMID: 11302691
2. Strub, GM. et al. (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25, 600-12. PMID: 20959514
3. Wei, Y. et al. (2017) Cell 168, 224-238.e10. PMID: 28017329
4. Yoshinaka, T. et al. (2019) iScience 19, 1065-1078. PMID: 31522117
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-PHB Polyclonal Antibody

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