Anti-Phosphoprotein Polyclonal Antibody (VK078014)

Name: Anti-Phosphoprotein Polyclonal Antibody
Brand: abinScience
SKU: VK078014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

VK078014

Description

Anti-Phosphoprotein Polyclonal Antibody (VK078014) is a rabbit polyclonal antibody detecting P phosphoprotein in ELISA, IHC, WB. Suitable for Nipah virus and Hendra virus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Nipah virus (NiV), Hendra virus (HeV)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant nipah virus Phosphoprotein (Asp471-Gly580).

Target

P phosphoprotein, partial; P/V/W/C

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O55778, Q9IK91

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Phosphoprotein is a ~78 kDa protein. Essential cofactor of the RNA polymerase L that plays a central role in the transcription and replication by forming the polymerase complex with RNA polymerase L and recruiting L to the genomic N-RNA template for RNA synthesis. Also plays a central role in the encapsidation of nascent RNA chains by forming the encapsidation complex with the nucleocapsid protein N (N-P complex). Acts as a chaperone for newly synthesized free N protein, so-called N0, allowing encapsidation of nascent RNA chains during replication. The nucleoprotein protein N prevents excessive phosphorylation of P, which leads to down-regulation of viral transcription/ replication. Participates, together with N, in the formation of viral factories (viroplasms), which are large inclusions in the host cytoplasm where replication takes place.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-P polyclonal antibody at 1ug/mL on various samples.
Lane 1: ATG16620 100ng

References

Datasheet

Anti-Phosphoprotein Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Phosphoprotein Polyclonal Antibody (VK078014)

Overview

Images

References

Datasheet

SDS

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