Anti-PIK3R1/PI3-kinase p85-alpha Polyclonal Antibody (HB055014)

Name: Anti-PIK3R1/PI3-kinase p85-alpha Polyclonal Antibody
Brand: abinScience
SKU: HB055014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HB055014

Description

Anti-PIK3R1/PI3-kinase p85-alpha Polyclonal Antibody (HB055014) is a rabbit polyclonal antibody detecting PI3-kinase subunit p85-alpha in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PIK3R1/PI3-kinase p85-alpha (Trp624-Val718).

Target

PI3-kinase subunit p85-alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, GRB1, PI3K regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PIK3R1, PI3-kinase regulatory subunit alpha

Purification

Purified by antigen affinity column.

Accession

P27986

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Phosphatidylinositol 3-kinase regulatory subunit alpha (PIK3R1/PI3-kinase p85-alpha) is a ~83 kDa protein. Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.

1. Miled, N. et al. (2007) Science (New York, N.Y.) 317, 239-42. PMID: 17626883

2. Mandelker, D. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 16996-7001. PMID: 19805105

3. Vainikka, S. et al. (1994) The Journal of biological chemistry 269, 18320-6. PMID: 7518429

4. Winnay, JN. et al. (2010) Nature medicine 16, 438-45. PMID: 20348923

Note

For research use only.

Images

References

Datasheet

Anti-PIK3R1/PI3-kinase p85-alpha Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PIK3R1/PI3-kinase p85-alpha Polyclonal Antibody (HB055014)

Overview

Images

References

Datasheet

SDS

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