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Anti-PITPNB Polyclonal Antibody (HW378014)

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Overview
Catalog No.HW378014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PITPNB (Met1-Val271).
Target Phosphatidylinositol transfer protein beta isoform, PtdInsTP beta, PI-TP-beta, PtdIns transfer protein beta, PITPNB
Purification Purified by antigen affinity column.
Accession P48739
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Phosphatidylinositol transfer protein beta isoform (PITPNB) is a ~31 kDa protein. Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes. Also catalyzes the transfer of sphingomyelin. Required for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum; phosphatidylinositol and phosphatidylcholine transfer activity is essential for this function.

1. Fullwood, Y. et al. (1999) The Journal of biological chemistry 274, 31553-8. PMID: 10531358
2. Shadan, S. et al. (2008) Traffic (Copenhagen, Denmark) 9, 1743-56. PMID: 18636990
3. Carvou, N. et al. (2010) Journal of cell science 123, 1262-73. PMID: 20332109
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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