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Anti-PLD1 Polyclonal Antibody (HC325014)

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Overview
Catalog No.HC325014
Description
Anti-PLD1 Polyclonal Antibody (HC325014) is a rabbit polyclonal antibody detecting PLD1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Cricetulus griseus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PLD1 (Thr725-Thr1074).
Target Choline phosphatase 1, PLD1, Phospholipase D1, PLD 1, Phosphatidylcholine-hydrolyzing phospholipase D1, hPLD1
Purification Purified by antigen affinity column.
Accession Q13393
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Phospholipase D1 (PLD1) is a ~124 kDa protein. Function as phospholipase selective for phosphatidylcholine. Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.

1. Yoon, MS. et al. (2015) Molecular cell 58, 549-56. PMID: 25936805
2. Hammond, SM. et al. (1995) The Journal of biological chemistry 270, 29640-3. PMID: 8530346
3. Lopez, I. et al. (1998) The Journal of biological chemistry 273, 12846-52. PMID: 9582313
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-PLD1 Polyclonal Antibody

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