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Anti-PNMT Polyclonal Antibody (HB552014)

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Overview
Catalog No.HB552014
Description
Anti-PNMT Polyclonal Antibody (HB552014) is a rabbit polyclonal antibody detecting Phenylethanolamine N-methyltransferase; PNMTase; 2.1.1.28; Noradrenaline N-methyltransferase; PNMT; PENT in ELISA, IHC, WB. Suitable for Human, Pig, Mouse, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human PNMT (Ser16-Leu282).
Target Phenylethanolamine N-methyltransferase; PNMTase; 2.1.1.28; Noradrenaline N-methyltransferase; PNMT; PENT
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P11086, Q06AU9, P40935, P10937, P10938
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Phenylethanolamine N-methyltransferase (PNMT) is a ~30 kDa protein. Catalyzes the transmethylation of nonepinephrine (noradrenaline) to form epinephrine (adrenaline), using S-adenosyl-L-methionine as the methyl donor. Other substrates include phenylethanolamine and octopamine. Also methylates normetanephrine.

1. Kang, DI. et al. (2010) Molecules and cells 29, 595-602. PMID: 20496117
2. Ji, Y. et al. (2005) Journal of neurochemistry 95, 1766-76. PMID: 16277617
3. Gee, CL. et al. (2005) Biochemistry 44, 16875-85. PMID: 16363801
4. Caine, JM. et al. (1996) Protein expression and purification 8, 160-6. PMID: 8812853
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-PNMT Polyclonal Antibody

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