Anti-POLD2 Polyclonal Antibody (HA706014)

Name: Anti-POLD2 Polyclonal Antibody
Brand: abinScience
SKU: HA706014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HA706014

Description

Anti-POLD2 Polyclonal Antibody (HA706014) is a rabbit polyclonal antibody detecting DNA polymerase delta subunit 2; DNA polymerase delta subunit p50; POLD2 in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human POLD2 (Met1-Pro469).

Target

DNA polymerase delta subunit 2; DNA polymerase delta subunit p50; POLD2

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P49005, P49004, O35654, Q6AXY4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA polymerase delta subunit 2 (POLD2) is a ~51 kDa protein. Accessory component of both the DNA polymerase delta complex and the DNA polymerase zeta complex. As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4.

1. Zhang, S. et al. (2007) The Journal of biological chemistry 282, 15330-40. PMID: 17317665

2. Chea, J. et al. (2012) Cell cycle (Georgetown, Tex.) 11, 2885-95. PMID: 22801543

3. Lee, YS. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 2954-9. PMID: 24449906

4. Xie, B. et al. (2002) Biochemistry 41, 13133-42. PMID: 12403614

5. Li, H. et al. (2006) The Journal of biological chemistry 281, 14748-55. PMID: 16510448

6. Meng, X. et al. (2009) Nucleic acids research 37, 647-57. PMID: 19074196

7. Meng, X. et al. (2010) Biochemistry 49, 3545-54. PMID: 20334433

8. Lin, SH. et al. (2013) DNA repair 12, 922-35. PMID: 24035200

9. Ogi, T. et al. (2010) Molecular cell 37, 714-27. PMID: 20227374

10. Costantino, L. et al. (2014) Science (New York, N.Y.) 343, 88-91. PMID: 24310611

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-POLD2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: 293T cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: HL-60 cell lysate
Lane 6: HepG2 cell lysate

References

Datasheet

Anti-POLD2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-POLD2 Polyclonal Antibody (HA706014)

Overview

Images

References

Datasheet

SDS