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Anti-POLR1H Polyclonal Antibody (HP567014)

Anti-POLR1H Polyclonal Antibody
Anti-POLR1H Polyclonal Antibody
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Overview
Catalog No.HP567014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human POLR1H (Met1-Ser126).
Target Zinc ribbon domain-containing protein 1, ZNRD1, RPA12, POLR1H, DNA-directed RNA polymerase I subunit H, DNA-directed RNA polymerase I subunit RPA12
Purification Purified by antigen affinity column.
Accession Q9P1U0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

DNA-directed RNA polymerase I subunit RPA12 (POLR1H) is a ~13 kDa protein. Core component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Can mediate Pol I proofreading of the nascent RNA transcript. Anchors into the Pol I active site to monitor transcription fidelity and cleave mis-incorporated 5'-ribonucleotides.

Note For research use only.
Images
  • Anti-POLR1H Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-POLR1H polyclonal antibody at 1ug/mL on recombinant POLR1H (Catalog No. HP567012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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