Anti-POLR2G Polyclonal Antibody (HA732014)

Name: Anti-POLR2G Polyclonal Antibody
Brand: abinScience
SKU: HA732014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA732014

Description

Anti-POLR2G Polyclonal Antibody (HA732014) is a rabbit polyclonal antibody detecting DNA-directed RNA polymerase II subunit RPB7; RNA polymerase II subunit B7; DNA-directed RNA polymerase II subunit G; RNA polymerase II 19 kDa subunit; RPB19; POLR2G; RPB7 in ELISA, IHC, WB. Suitable for Rat, Human, Mouse, Bovine, and Zebrafish.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human POLR2G (Met1-Ser172).

Target

DNA-directed RNA polymerase II subunit RPB7; RNA polymerase II subunit B7; DNA-directed RNA polymerase II subunit G; RNA polymerase II 19 kDa subunit; RPB19; POLR2G; RPB7

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P62489, P62487, P62488, Q5E9B8, Q7ZW41

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA-directed RNA polymerase II subunit RPB7 (POLR2G) is a ~19 kDa protein. Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2G/RPB7 is part of a subcomplex with POLR2D/RPB4 that binds to a pocket formed by POLR2A/RPB1, POLR2B/RPB2 and POLR2F/RPABC2 at the base of the clamp element. The POLR2D/RPB4-POLR2G/RPB7 subcomplex seems to lock the clamp via POLR2G/RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-POLR2G polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Lane 4: A375 cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: U251 cell lysate
Lane 7: SW620 cell lysate
Lane 8: A549 cell lysate

References

Datasheet

Anti-POLR2G Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-POLR2G Polyclonal Antibody (HA732014)

Overview

Images

References

Datasheet

SDS

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