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Anti-POLR2J Polyclonal Antibody (HB324014)

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Overview
Catalog No.HB324014
Description
Anti-POLR2J Polyclonal Antibody (HB324014) is a rabbit polyclonal antibody detecting DNA-directed RNA polymerase II subunit RPB11-a; RNA polymerase II subunit B11-a; RPB11a; DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit; POLR2J; POLR2J1 in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human POLR2J (Met1-Glu117).
Target DNA-directed RNA polymerase II subunit RPB11-a; RNA polymerase II subunit B11-a; RPB11a; DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit; POLR2J; POLR2J1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q32P79, P52435, O08740, Q9H1A7, Q9GZM3
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

DNA-directed RNA polymerase II subunit RPB11 (POLR2J) is a ~13 kDa protein. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2J/RPB11 is part of the core element with the central large cleft.

Note For research use only
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Formula
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V₁ (Stock Vol.)
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Anti-POLR2J Polyclonal Antibody

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