Anti-Porphyromonas gingivalis kgp/prtK Polyclonal Antibody (JN089014)

Name: Anti-Porphyromonas gingivalis kgp/prtK Polyclonal Antibody
Brand: abinScience
SKU: JN089014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

JN089014

Species reactivity

Porphyromonas gingivalis

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Porphyromonas gingivalis kgp/prtK (Asp229-Pro683).

Target

Lys-gingipain W83, 3.4.22.47, Lysine specific cysteine protease, Lysine-specific cysteine proteinase, Porphypain, PrtK48, Lys-gingipain catalytic subunit, 39 kDa adhesin, PrtK39, 15 kDa adhesin, PrtK15, 44 kDa adhesin, PrtK44, kgp, prtK, prtP

Purification

Purified by antigen affinity column.

Accession

Q51817

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Lys-gingipain W83 (kgp/prtK) is a ~187 kDa protein. Cysteine proteinase with a strong preference for substrates with Lys in the P1 position. Hydrolyzes bovine hemoglobin, bovine serum albumin, casein, human placental type I collagen and human IgA and IgG. Disrupts the functions of polymorphonuclear leukocytes. May act as a virulence factor in the development of peridontal disease. Involved in the coaggregation of P.gingivalis with other oral bacteria.

1. Bhogal, PS. et al. (1997) Microbiology (Reading, England) 143 ( Pt 7), 2485-2495. PMID: 9245829

2. Li, N. et al. (2011) Molecular microbiology 81, 1358-73. PMID: 21812842

3. Ganuelas, LA. et al. (2013) European journal of microbiology & immunology 3, 152-62. PMID: 24265933

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Kgp polyclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 70 ug
Lane 2: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 50 ug
Lane 3: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 30 ug
Lane 4: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 10 ug

References

Datasheet

Anti-Porphyromonas gingivalis kgp/prtK Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Porphyromonas gingivalis kgp/prtK Polyclonal Antibody (JN089014)

Overview

Images

References

Datasheet

SDS

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