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Anti-POU2AF1 Polyclonal Antibody (HA822014)

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Overview
Catalog No.HA822014
Description
Anti-POU2AF1 Polyclonal Antibody (HA822014) is a rabbit polyclonal antibody detecting POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1; POU2AF1; BOB1; OBF1 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human POU2AF1 (Leu2-Phe256).
Target POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1; POU2AF1; BOB1; OBF1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q16633, Q2KJA4, Q64693
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

POU domain class 2-associating factor 1 (POU2AF1) is a ~27 kDa protein. Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2. It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator.

1. Strubin, M. et al. (1995) Cell 80, 497-506. PMID: 7859290
2. Gstaiger, M. et al. (1995) Nature 373, 360-2. PMID: 7779176
3. Luo, Y. et al. (1995) Molecular and cellular biology 15, 4115-24. PMID: 7623806
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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V₁ (Stock Vol.)
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Anti-POU2AF1 Polyclonal Antibody

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